Emulsion PCR Protocol

Monosized, paramagnetic, streptavidin-coated MyOne beads are pre-loaded with a dual biotin forward primer. An aqueous phase is prepared with the above beads, low-cost PCR polymerase, PCR buffers and dNTPs, and template DNA. This is mixed with an oil phase, and vortexed to create the emulsion. Each droplet will ideally have one bead, and one molecule of template DNA. PCR is then performed to amplify the template DNA within each emulsion droplet. A simple device to implement the emulsion PCR protocol will be available for sale in 2009.